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The sample size was calculated based on the findings from similar studies at 95% confidence interval. The exclusion criteria excluded subjects (1) with marked intraoral soft tissue pathology, (2) on an antibiotic regimen 3 months before or during the course of study, (3) with medically compromising conditions, (4) undergoing orthodontic therapy.
#How to calculate ppm fluoride full
The inclusion criteria required subjects with the following : (1) A full complement of teeth (except for third molars and premolars extracted for orthodontic therapy) (2) DMFT score less than one (3) Mild or no gingivitis with an acceptable attachment loss of less than 2 mm (4) No evidence of a deeply fissured tongue or irregular oral mucosal surfaces which might enhance fluoride retention (5) Unstimulated salivary flow rates between 0.2 and 0.7 mL/min The sample consisted of 60 adolescents between 16 and 18 years.
#How to calculate ppm fluoride trial
The triple blinded randomized parallel group trial has been reported in accordance with the CONSORT 2010 guidelines. The null hypothesis states that no difference exists in the saliva and plaque F concentration following dentifrice application. The present study aims to assess plaque and salivary F levels at different time intervals up to 24 hours after the use of high fluoride dentifrice in varying concentrations in an optimally fluoridated community. However, the comparison of fluoride levels in saliva and plaque has not been explored following use of dentifrices with high fluoride concentrations.
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Most of the clinical studies evaluating these toothpastes have focused on caries reduction. ĭentifrices with higher concentrations of fluoride (28 ppm) have recently been introduced but are available only on prescription. Fluoride toothpastes with <1450 ppm F content have been reported to be less effective in high-risk children. suggested that an increase of 500 ppm F (within the range of 1100–2500 ppm F) would amplify caries reduction potential by 6%. The higher the fluoride concentration available is, the greater its impetus to diffusion through the biofilm towards the tooth surface will be. The caries protection from fluoride (F) dentifrices is largely dependent on the efficiency of the fluoride delivery and its ability to increase intraoral fluoride levels. Among the available fluoride delivery systems, dentifrices are most commonly used. This has led to a dramatic decline in the prevalence of dental caries globally. Since the discovery of its anticariogenic potential, fluorides have been at the forefront of preventive dentistry. Both high fluoride dentifrices were effective in maintaining higher intraoral fluoride levels up to 12 hours and 5000 ppm up to 24 hours compared to conventional dentifrice.
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There was a positive correlation between fluoride levels in saliva and plaque. A significant difference in fluoride levels was observed at different time intervals in both saliva and plaque among the three groups. The variance was low with time and concentration of the dentifrice used in saliva, whereas the magnitude of treatment was large for time (>0.25) but small for (<0.09) concentration in plaque. A significant difference in fluoride levels was observed at different time intervals in both saliva (p=0.048) and plaque (p=0.03). Data was collected and statistically analyzed using SPSS 20 Inc. Fluoride levels were determined using SPADNS method. Unstimulated whole saliva and plaque samples were collected at different time intervals. Subjects were asked to brush the occlusal surfaces of posterior teeth with one of the dentifrices for 2 min. This randomised control trial was conducted on sixty adolescents between the age group of 16 and 18 years divided into three groups A, B, and C.
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The aim of the present study was to assess salivary and plaque fluoride levels at different time intervals following the use of high fluoride dentifrices. The comparison of fluoride levels in saliva and plaque following the use of conventional, 28 ppm dentifrices for different time intervals up to 24 hours has not been explored.